Short-term toxicity of Orange G in pigs.

Abstract--Orange G was given to groups of three male and three female pigs for 112 days at doses of 0 (control), 2"5, 25 or 250 mg/kg/day. No adverse effects were found in pigs given 2"5 mg Orange G/kg/day. At the higher levels, Heinz bodies were found in the erythrocytes, together with anaemia, reticulocytosis, splenomegaly and, at the highest level only, a histologically demonstrable increase in splenic iron. The no-untoward-effect level in this study was 2.5 mg/kg/day.